Ph AP protease from Pseudoalteromonas haloplanktis TAC125: Gene cloning, recombinant
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概要
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南極の海洋性バクテリアであるPseudoalteromonas haloplanktis TAC125により生産される細胞外酵素について、タンパク質分解酵素の低温適応を調べた。プロテオミクス解析により、その培養液の上澄みからいくつかのタンパク分解活性を同定した。以後の解析にはPhAPプロテアーゼを選び、その遺伝子をクローニングして遺伝子組み換え技術を用い、大腸菌細胞中で生産させた。得られたプロテアーゼの生化学的特徴を調べたところ、酵素は亜鉛依存型のアミノペプチドであり、その活性は典型的な好冷性酵素の特徴を示すことが明らかになった。
著者
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D. de
Institute of Protein Biochemistry, CNR, Via Pietro Castellino 111, I-80131 Naples, Italy
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M. Giuliani
Department of Organic Chemistry and Biochemistry, University of Naples Federico II, Complesso Univer
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C. De
Institute of Protein Biochemistry, CNR, Via Pietro Castellino 111, I-80131 Naples, Italy
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N. Bergamasco
Institute of Protein Biochemistry, CNR, Via Pietro Castellino 111, I-80131 Naples, Italy
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A. Amoresano
Department of Organic Chemistry and Biochemistry, University of Naples Federico II, Complesso Univer
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A. Carpentieri
Department of Organic Chemistry and Biochemistry, University of Naples Federico II, Complesso Univer
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E. Parrilli
Department of Organic Chemistry and Biochemistry, University of Naples Federico II, Complesso Univer
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M.L. Tutino
Department of Organic Chemistry and Biochemistry, University of Naples Federico II, Complesso Univer
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M. Giuliani
Department Of Organic Chemistry And Biochemistry University Of Naples Federico Ii Complesso Universi
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A. Amoresano
Department Of Organic Chemistry And Biochemistry University Of Naples Federico Ii Complesso Universi
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A. Carpentieri
Department Of Organic Chemistry And Biochemistry University Of Naples Federico Ii Complesso Universi
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M.l. Tutino
Department Of Organic Chemistry And Biochemistry University Of Naples Federico Ii Complesso Universi
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C. De
Institute Of Protein Biochemistry Cnr Via Pietro Castellino 111 I-80131 Naples Italy
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E. Parrilli
Department Of Organic Chemistry And Biochemistry University Of Naples Federico Ii Complesso Universi
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N. Bergamasco
Institute Of Protein Biochemistry Cnr Via Pietro Castellino 111 I-80131 Naples Italy
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D. De
Institute Of Protein Biochemistry Cnr Via Pietro Castellino 111 I-80131 Naples Italy
関連論文
- Ph AP protease from Pseudoalteromonas haloplanktis TAC125: Gene cloning, recombinant
- PhAP protease from Pseudoalteromonas haloplanktis TAC125: Gene cloning, recombinant production in E. coli and enzyme characterization