分泌型白血球プロテアーゼインヒビターによる歯肉上皮細胞のPorphyromonas gingivalis感染制御
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Secretory leukocyte protease inhibitor (SLPI) has been recognized as not only a protease inhibitor but also an important defense component in mucosal secretory fluids. To elucidate the functional role in innate immunity in gingival crevices, the SLPI production from a gingival epithelial cell line, GE1, with or without the stimulation of Porphyromonas gingivalis lyophilized whole cells (Pg-WC) and the lipopolysaccharides (Pg-LPS), and the inhibitory effect on P. gingivalis proteases were investigated. The unstimulated GE1 cells showed low levels of SLPI mRNA expression, which was augmented by the stimulation with Pg-LPS as well as Pg-WC. The augmentation of SLPI mRNA expression in GE1 cells was accompanied by the inductions of IL-6, TNF-α and IL-1β mRNA expressions. Although IL-6 could induce macrophages to produce SLPI, the kinetics analyses suggested that the augmentation of SLPI production in GE1 cells could not be a second response to the IL-6 induced by the stimulant, but a direct response by the P. gingivalis antigens. Further experiments using rSLPI indicated that SLPI showed a direct inhibitory effect on the P. gingivalis protease of Lys-gingipain. Thus, it was suggested that gingival epithelial cells could be a substantial producer of SLPI that functions inhibitory to the pathogenic P. gingivalis protease in gingival crevices. It was also suggested that the SLPI production could increase in response to P. gingivalis through the stimulation with its pathogenic constituents.
- 2010-04-23
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