L-Arginine and L-Ornithine Supplementation Facilitates Angiogenesis and Causes Additional Effects on Exercise-induced Angiogenesis in Hind-leg Muscles
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The present study was designed to examine the effects of dietary L-arginine and L-ornithine supplementation with or without exercise on capillary angiogenesis in rats. Exercise training lasted for six weeks at 20m/min on a 15% gradient for 15-60min/day. Rats in the treated groups drank water containing 4% L-arginine and 2% L-ornithine. According to histochemical identification of the capillary profile, the supplementation significantly increased the capillary-to-fiber (C:F) ratio in mixed fiber portion of the plantaris muscle. In the soleus and superficial portion of plantaris muscles, training with supplementation significantly increased the C:F ratio, whereas training alone did not. Western blot analysis showed that training with or without supplementation significantly enhanced VEGF protein expression in the soleus muscle, while training with supplementation enhanced it significantly in the plantaris muscle. The supplementation with or without training significantly increased tissue levels of eNOS in the soleus muscle (1.5-fold and 1.7-fold, respectively), while training alone increase it non-significantly. Supplementation significantly lowered endostatin protein levels (0.58-fold) in the plantaris muscle. The present results suggest that supplementation facilitates angiogenesis by inhibiting antiangiogenic endostatin expression in the skeletal muscle that composed of mixed fiber-type distribution, and it causes additional effects on exercise-induced angiogenesis possibly by promoting a NO/VEGF interrelationship in skeletal muscle that are mainly composed of slow-twitch fibers.
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関連論文
- L-Arginine and L-Ornithine Supplementation Facilitates Angiogenesis and Causes Additional Effects on Exercise-induced Angiogenesis in Hind-leg Muscles
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