胎生初期に血管内皮細胞に限局して発現する遺伝子del-1の同定と解析

概要

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We have taken advantage of an enhancer-trap event in a line of transgenic mice to study a unique developmentally regulated endothelial cell locus (del-1). Studies employing the lac-Z reporter transgene indicated that early expression of this locus was restricted to endothelial cells and their progenitors, with expression in later development also being found in hypertrophic chondrocytes, retinal neurons, and other cells known to regulate vascular formation. Characterization of the del-I gene and the protein encoded in this locus was accomplished by genomic and CDNA cloning. Deduced amino acid sequence indicates that Del-1 is a secreted protein which contains three epidermal growth factor (EGF)-like repeats and two Discoidin-like domains. The second EGF-like repeat contains an Arg-Gly-Asp recognition sequence. Western blot and immunohistochemical studies indicate that Del-1 is deposited primarily in the extracellular matrix, where it likely functions to regulate vascular formation.
東京女子医科大学の論文
1996-10-25