p53蛋白の発現と超微形態からみた声帯白斑症の診断と悪性度について

概要

論文の詳細を見る
Twenty five cases of vocal cords leukoplakia, known as precancerous lesion, were classified into three degrees of dysplasia and examined for the mutant p53 protein immunohistochemically to correlate its expression with the degree of dysplasia. Mutation of the tumor suppressor gene p53 plays a role in the multi step processes of tumorigenesis. Two of the 9 cases without dysplasia, 3 of the 10 cases with mild dysplasia, one of the 3 cases with moderate dysplasia, and 3 of the 3 cases with severe dysplasia, and 2 of the 2 cases of vocal cords carcinoma gave positive immunoreactivity to the mutant p53, respectively. Transmission electron microscopy revealed that even in the cases with mild dysplasia, loosening the cell to cell contact accompanied by loss of keratin and desmosome, and rounding of the nucleus with loss of heterochromatin were already manifest although the nucleus to cytoplasm ratio were still within the normal range. In fact, 60% of the patients with the vocal cords leukoplakia classified as mild dysplasia suffered a relapse after CO_2 laser therapy. Thus, the ultastructural change of the sub cellular organelle may be a useful maker for diagnosis of vocal cords leukoplakia. However, expression of the mutant p53 protein dose not seem to correlate with the degree of dysplasia and screening with this marker may overlook the potential malignancy of the precancerous lesion.
東京女子医科大学の論文
1997-10-25