ベーチェット病患者における多核白血球の補体第3成分(C3)産生能

概要

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To elucidate the relationship between the functions of polymorphonuclear leukocytes (PMN) and the complement system in patients with Behcet's disease, we analyzed peripheral PMN and the following phenomena were assessed: (1) production of the third component of complement (C3), (2) relationship between secretion and expression of mRNA of C3, (3) effect of 12-o-tetradecanoylphorbol 13-acetate (TPA) on C3 production. The PMN samples were purified more than 98% by the double gradient method and were incubated for 24 hours. C3 levels in culture supernatants were quantitated by sandwich enzyme-linked immunosorbent assay, and C3 mRNA were detected by a reverse transcription-polymerase chain reaction assay and Southern blot hybridization. C3 secretion by Behcet's PMN was significantly higher than that of normal PMN, though the expression of C3 mRNA by Behcet's PMN was almost the same as that of normal PMN. In the Behcet's PMN group, C3 secretion is closely related to the time-course of ocular attack. When TPA was added to the culture supernatant, C3 secretion and C3 mRNA expression by normal PMN were somewhat increased, while there were no changes in the Behcet's PMN. These observations indicate that Behcet's PMN are primed in vivo and suggest that C3 produced by PMN plays a role in the pathophysiology of Behcet's disease.
1997-02-25