ベーチェット病患者リンパ球におけるFas/Fasリガンドシステムの発現に関する検討

概要

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The expression of Fas antigen, Fas sensitivity, soluble Fas (sFas) and soluble Fas ligand (sFasL) levels were examined to investigate the role of the Fas/Fas ligand system in the pathogenesis of Behget Disease (BD). The expression of Fas antigen was examined by flow cytometry, and the Faspositive rate of peripheral blood mononuclear cells in BD patients (n=12) was significantly higher than that in normal controls (NCsi n=8, p<0.05). Furthermore, the Fas-positive rate was significantly suppressed in BD patients who used immunosuppressants (n=10, p<0.05). The Fas sensitivities of peripheral T cells to anti-Fas monoclonal antibody were evaluated by cell counting and trypan blue exclusion. The sensitivities in BD patients (n=12) was significantly increased compared to those in NCs (n=12, p<0.005). Quantification of sFas and sFasL was performed by the sandwich ELISA method. The sFas level of BD patients in the inactive stage (3.74 ng/ml, n=38) was significantly increased compared to the early-active stage (2.14 ng/ml, n=17) and to NCs (1.93 ng/ml, n=15). No significant difference in sFasL levels was found between the stages of BD and NCs. Some disorders of Fas/Fas ligand system in BD were revealed in this study, which may pertain to the inflammatory mechanism in BD.
東京女子医科大学の論文
1999-01-25