STUDIES ON THE ANTIGENICITY OF THE HUMAN TROPHOBLAST CELL BY MICRO MIXED LEUKOCYTE-TROPHOBLAST CELL CULTURE
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概要
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Mixed leukocyte-trophoblast cell cultures (MLTC) were used in 37 cases of normal trophoblast cells and in 25 cases of spontaneously aborted trophoblast cells to determine whether or not trophoblast cells have the ability to express histocompatibility antigens. In addition, the author attemped to investigate the differences in the histocompatibility antigenicity amoung the trophoblast cell, the fetal call and from normal helathy adult. Furthermore, mixed wife-husband leukocyte cultures were set up from the samples of 13 couples with a history of habitual abortion and 13 normal couples, in order to investigate the role of immunologic factors, and the Stimulation Index (S.I.) was calculated. 1) The mean cpm of mixed maternal leukocyte-normal trophoblast cell culture and maternal leukocyte solo culture were 822±479 cpm and 427±479 cpm, respectively. The difference was statistically significant (P<0.01). 2) The trophoblast cell had the least antigenicity to the maternal leukocyte compared with the fetal cell and leukocyte from a normal healthy adult. 3) The mean S.I. of mixed maternal leukocyte-spontaneously aborted trophoblast cell culture was significantly higher than that obtained by using a normal trophoblast cell as the stimulating cell (P<0.01). 4) In cases of habitual abortions, the MLC reaction was higher than those in normal couples, when the wife's leukocyte was used as the responding cell and the husband's leukocyte as the stimulating cell in either group.
- 1978-08-28