STUDIES ON FORMATION OF LIPID PEROXIDE IN THE PREMATURE RETINA BY OXYGEN ADMINISTRATION AND/OR LIGHT IRRADIARTION, AND THE EFFECT OF ANTIOXIDANTS ON LIPID PEROXIDE
スポンサーリンク
概要
- 論文の詳細を見る
It is well known that retinopathy of prematurity develops on the basis of prematurity of the retina, and that lipid peroxide is increased in various tissues by the biochemical reaction of oxygen and/or light. Recently, a few biochemists have proposed that lipid peroxide formed in the blood and retina by oxygen and/or light is partly responsible for the occurrence and advance in retinopathy of prematurity. In this experiment, the author administered oxygen and/or light to the retina of chick embryos on day 14 of gestation and to that of mature chicks 10 days after hatching in vitro. Then the level of lipid peroxide in the retina was measured by a modified TBA fluorometric assay. Since vitamin E acts as anantioxidant which inhibits the formation of lipid peroxide in the tissue, the vitamin E level was also measured in the embryonic and mature specimens by using a high speed liquid chromatographic (HSLC) method with a spectrofluorometer. In addition, the effect of dl-α-tocopherol or riboflavin-2',3',4',5'-tetrabutyrate (B_2-But) on lipid peroxide in the retina of the chick embryos on day 14 of gestation in vitro was investidated. The results were as follows. 1. It was evident that the level of lipid peroxide in the premature retina was greater than in the mature retina, even when there was neither oxygen administration nor light irradiation (0.05<p<0.1). 2. In both the premature and mature retina, oxygen plus light had the greatest debilitative effect. Oxygen alone had the second greatest effect, followed by light alone. These effects were more pronounced in the premature retina than in the mature retina (p<0.05). 3. The vitamin E level in the retina of chick embryos on day 14 of gestation was 0.22 μg/g wet weight (S.D.±0.05 μg/g wet weight), while that in the retina of chicks 10 days after hatching was 6.81 μg/g wet weight (S.D.±2.15 μg/g weight). There was the significant difference between these two levels (p<0.001). 4. The coexistence of riboflavin-2',3',4',5'-tetrabutyrate (B_2-But) or dl-α-tocopherol inhibited the formation of lipid peroxide. In particular, dl-α-tocopherol markedly decreased the lipid peroxide level. B_2-But decomposed lipid peroxide following light irradiation , whereas dl-α-tocopherol did not. From these results, the author concluded that the formation of lipid peroxide may be one of the most important causes of retinopathy of prematurity.
- 名古屋市立大学の論文
- 1979-11-30