CHANGES IN SERUM LIPOPROTEIN IN HYPOVITAMIN C ATHEROSCLEROSIS
スポンサーリンク
概要
- 論文の詳細を見る
Effects of hypovitamin C on serum lipoproteins were studied in guinea pigs in which chronic hypovitamin C was induced by a scorbutigenic diet (Group 1) or 1% cholesterol and 5% coconut oil-added scorbutigenic diet (Group 3) for two weeks. The controls were fed on a similar diet supplemented with 25 mg of vitamin C daily (Group 2 and 4). Lipoproteins were isolated by ultracentrifugation according to Hatch's method. Cholesterol, triglyceride, phospholipid, and protein contents in isolated lipoprotein fractions were estimated with colorimetric methods. In Group 1, amounts of chylomicron, very low density lipoprotein (VLDL), low density lipoprotein (LDL), and high density lipoprotein (HDL) classes were significantly elevated as compared to the control (Group 2). Concentrations of tryglyceride in VLDL, and cholesterol ester in LDL were also increased in Group 1. Increased LDL, lower cholesterol and higher apoprotein levels in HDL were obserbed in Group 3 as compared with the control (Group 4). Eight bands of apoprotein in HDL were separated by polyacrylamide gel disc electrophoresis in the presence of Triton X-100. The first band of Group 1 (supposedly arginin-rich peptide) was very darkly stained and the others (supposedly Apo A and Apo C groups) were faintly stained compared with those of Group 2. The first band of Group 3 was also very darkly stained as in Group 1. The staining intensity of the other bands of Group 3 as decreased as compared with that of the control (Group 4). From these results, imapaired clearance of chylomicron remnants and accumulation of cholesterol ester-rich chylomicron and LDL by hypo vitamin C were assumed. It is suggested that hypovitamin D induces an alteration of the lipoprotein metabolism resulting in change of the lipid composition and apoprotein constituents of the lipoprotein. Thus, hypovitamin C is very possibly a factor in the pathogenesis of atherosclerosis not only in virtue of the decreases integrity of the vascular wall, as previously reported, but also due to the altered lipid metabolism ad observed in the present study.
- 名古屋市立大学の論文
- 1979-11-30