NATURAL CELL-MEDIATED CYTOTOXICITY IN MAN
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概要
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N cells responsible for natural cell-mediated cytotoxicity (NCMC) were revealed to be neither conventional T nor B cells but Fc receptor-bearing cells, suggesting that N cells in NCMC and K cells in antibody-dependent cell-mediated cytotoxicity (ADCC) were similar. When lymphocytes were treated for 30 min at 37℃ with trypsin, N cell activity was signigicantly inhibited whereas ADCC was unaffected. When protein A, which binds selectively to the Fc portion of IgG, was added to the ^<51>Cr release assay, ADCC was significantly inhibited, presumably due to binding of protein A to available Fc sites of the sensitizing antibody. Protein A, however, had no effect on N cell reactivity. These results indicate that effector cells in NCMC and ADCC are in overlapping and the cytolytic mechanisms in NCMC and ADCC appear to be distinct. Application of cross-competition assay (CCA) to NCMC revealded by T cells in response to mitomycin C-treated lymphoblastoid cell lines. There appeared to be no restriction for compatibility at the A and B locus of HLA antigens. The factor also increased the activity of K cells in ADCC further supporting the concept that N and K cells are identical. NAF may be differentiated from two other lymphokines in the supernatant of mixed cultures, lymphotoxin and an inhibitor of DNA synthesis (IDS), by temperature sensitivity and column chromatography. It is a heat-labile nondialyzable protein that is eluted from Sephadex G-100 after serum alubumin.
- 名古屋市立大学の論文
- 1980-03-30