ARGININE VASOPRESSIN LEVELS IN PLASMA DURING NORMAL PREGNANCY AND LABOR

概要

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A sensitive and specific radioimmunoassay (RIA) for arginine vasopressin (AVP) is described. The method involved careful collection and rapid centrifugation of the sample, extraction with Florisil, and RIA using ^<125>I-AVP and the antisera produced in rabbits immunized against AVP conjugated to bovine serum albumin (BSA). The antisera showed insignificant cross-reaction with small peptides including oxytocin, lysine vasopressin (LVP) and other pituitary peptides. The limit of detection of the assay was 1.0 pg/assay tube with intra-assay also introduces blood sampling and plasma preservation methods to avoid the degradation by systine aminopeptidase (CAP) which is present in high concentration in the plasma of pregnant women. Pregnant women at unrestricted fluid intake showed a wide range of AVP levels not differing from those in non-pregnant women. Serial samples of material plasma showed no significant increase in AVP during the various stages of labor though a slight rise was observed during the second stage of labor. AVP concentrations in the umbilical arterial plasma were significantly high in labor. The significance of these findings is discussed.
名古屋市立大学の論文
1981-06-04