INCREASED ACTIVITY OF CATHEPSIN D AND CATHEPSIN E-LIKE ENZYME IN RAT SPLEEN BY PHENYLHYDRAZINE ADMINISTRATION
スポンサーリンク
概要
- 論文の詳細を見る
The body weight, protein contents and weight of liver and spleen, reticulocyte counts in blood, and acid phosphatase and cathepsin D activities in anemic rat liver and spleen were carefully measured after a single of continuous subcutaneous administration of phenylhydrazine hydrochloride (PHH) at a dose of 2.5 mg/100 g body weight. Cathepsin D activity in the liver and especially in the spleen increased with the PHH injection, while acid phosphatase activity showed no signigicant change. Cathepsin D in spleen was separated from cathepsin E-like enzyme on Sephadex G-100 column chromatography. These two acid proteinases were remarkably changed with the progress of hemolytic anemia. Cathepsin E-like enzyme appeared rapidly after the PHH injection, and seemed to be correlated with the formation of splenomegaly. On the other hand, cathepsin D gradually increased with the PHH injection, and thid seemed to be correlated with the remission of splenomegaly. After PHH injection, when spleen cells were fractionated with the use of Ficoll-Paque, the granulocyte-rich fraction retained its marked cathepsin E-like enzyme activity. These results demonstrate that two similar proteinases in spleen, cathepsin D and cathepsin E-like enzyme, were characterized with the PHH injection, and that cathepsin D plays an important role in the degradation of phenylhydrazine-damaged erythrocytes in rat, whereas the cathepsin E-like enzyme was less effective in this process. The increased activity of cathepsin E-like enzyme resulted from the accumulation of granulocytes in spleen.
- 名古屋市立大学の論文
- 1983-12-23