Expression and purification of the recombinant mustard trypsin inhibitor 2 (MTI2) in Escherichia coli(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

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概要

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• The mustard trypsin inhibitor 2, MTI2, was expressed in Escherichia coli. A specific procedure for its production and purification is described. The recombinant protein was recovered by protein extraction from the insoluble fraction, then renatured and purified by ion exchange and gel filtration chromatography. Finally, the inhibitory activity against trypsin was also determined.

• 社団法人日本生物工学会の論文

著者

• Stefan Alessandra

  Department of Industrial Chemistry, University of Bologna

• Hochkoeppler Alejandro

  Department of Industrial Chemistry, University of Bologna

• Stefan Alessandra

  Department Of Industrial Chemistry University Of Bologna:csgi Department Of Chemistry University Of

• Hochkoeppler Alejandro

  Department Of Industrial Chemistry University Of Bologna:csgi Department Of Chemistry University Of

• Ugolini Luisa

  Agricultural Research Council Research Centre For Industrial Crops

• Martelli Elena

  Department of Industrial Chemistry, University of Bologna

• Palmieri Sandro

  Agricultural Research Council, Research Centre for Industrial Crops

• Martelli Elena

  Department Of Industrial Chemistry University Of Bologna

• Palmieri Sandro

  Agricultural Research Council Research Centre For Industrial Crops

関連論文

• Shine-Dalgarno sequence enhances the efficiency of lacZ repression by artificial anti-lac antisense RNAs in Escherichia coli(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Expression and purification of the recombinant mustard trypsin inhibitor 2 (MTI2) in Escherichia coli(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

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