実験的脱髄性脳脊髄炎における病理組織学的,組織化学的研究
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概要
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The present investigation is concerned with demyelination as a chemical reaction in tissue. Experimental demyelinating encephalomyelitis in guinea pigs and rabbits was studied by using shistopathological and histochemical techniques. The materials consisted of 24 guinea pigs and 10 rabbits all injected with antigen and adjuvants in accordance with Freund's technique. Only 8 of the guinea pigs developed demyelination, while almost all of animals produced various histopathological changes. The results obtained are summarized as follows : 1) The histopathological findings in the brain of guinea pigs were the lymphocytic infiltration and the adventitial cell proliferation around the blood vessels in the chorioid plexus together with perivascular demyelinated foci near the lateral ventricule. The most characteristic finding in the brain of guinea pigs was the change in the mesenchymal tissue, and the cerebrospinal fluid had something to do with this change. 2) In regard to the histochemical findings, the fatty products could not be recognized in the fresh demyelinated lesions. The substance which showed positive reaction in PAS and metachromasia was seen in the protoplasm of numerous cells about the center of the lesions. On the contrary, the fatty products which were the phosphatide were seen in the protoplasm of a few cells in the periphery of the older lesions. In the periphery of the lesions the number of cells which had PAS positive and metachromatic substance in their bodies was decreased. From the result of tetrazonium reaction, the PAS positive and metachromatic substance seemed to be combined with protein. 3) The activity of alkaline phosphatase increased gradually in the tissue in relation to the number of injections of antigen. It was decreased in the small demyelinated lesions and lost in the large lesions. The activity of acid phosphatase strongly increased in the demyelinated lesions. These abnormal reactions were located mainly around the blood vessels in the small lesions, but they extended throughout the histological focal area in the large lesions. It seemed to us that the activity of acid phosphatase is related to the demyelination and that the activity of acid phosphatase replaced the activity of alkaline phosphatase in the process of demyelination. 4) Considering the facts studied histochemically, it is very interesting to note that the changes of fats and phosphatases in demyelination bear a striking resemblance to the results of chemical investigation in Wallerian degeneration.
- 1963-11-28