放線菌の産生する蛔虫角皮溶解物質N-63の研究

概要

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Four strains among 320 isolates of soil streptomyces were found to produce active principles digesting ascaris cuticle. S. albus N-63 showed the strongest activity and the isolation of the active principle from the culture broth was carried out. Fourty eight hours' culture broth of S. albus N-63 was filtered, concentrated by freezing, and fractionary precipitated with alcohol. The precipitate was then dissolved in water. The active principle was further salted out by (NH_4)_2SO_4 soln., again dissolved in water, and dialyzed against running water. The purified material exhibited high activity of digesting heated cuticle of pig ascaris at the concentration of 7.8 mcg/ml after 48 hours. High concentration of the substance, i. e. 2 to 10 mg/ml, digested the cuticle completely in 20 minutes. The optimal pH was found to be around 7.0. The substance also showed about the same activity against live pig ascaris as that of ficin protease. The mode of action, however, was a little different from each other. Disturbed and abnormal movement of ascaris was observed in U-form tube filled with Locke's solution containing this active substance.
千葉大学の論文