オキシ安息香酸及びその誘導体の定量とその代謝

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The present paper deals with the metabolism of hydroxybenzoates derivatives in vitro and modifications of the determinations of these compounds. 1) The concentrations of o-, and m-hydroxybenzoic acid in clear deproteinized filtrate are determined with spectrophotometer at 310 mp and 300 mp respectively. 2) Salicylic acid and salicyluric acid present together in a mixture are determined seperately; the former in the extract with chloroform at 300 mi and then the latter in the succesive extract with ether at the same wavelength. 3) p-Hydroxybenzoic acid and p-hydroxyhippuric acid in the test solution are determined seperately at 260 mp as described above; after extracting the former with the solvent mixture of ether and petroleum ether, and then the latter with isoamylalcohol. 4) Salicylamide, salicyluric acid, salicylalanine, salicylleucine, salicylglutamic acid are determined by the indophenol method of Akamatsu and Shishikura, after extracting these compounds with organic solvents as follows to separate from interfering substances that may inhibit the color reactions: Salicylamide, salicylalanine and salicylleucine are extracted with chloroform, salicyluric acid with n-butanol-benzene mixture, and salicylglutamic acid with ethylacetate. Acetoxybenzoate was hydrolysed by almost all the organ homogenates in the order of p-> m-> o- compounds. Salicylamide, salicyluric acid, salicylalanine, salicylleucine, and salicylglutamic acid were not hydrolysed enzymatically. Synthesis of salicyluric acid or p-hydroxyhippuric acid from glycine salicylate or glycine p-hydroxybenzoic abid was not achieved in vitro. Hydroxy group of hydroxy-benzoate seems to inhibit the synthesis of their glycine conjugates.
千葉大学の論文

オキシ安息香酸及びその誘導体の定量とその代謝

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