齲食活動性試験に及ぼす諸因子の細菌学的化学的研究
スポンサーリンク
概要
- 論文の詳細を見る
One of our co-worker, M. Tanabe investigated the correlation between the Snyder test and the susceptibility of caries, and ascertained the relationship between the result of Snyder test and the number of salivary bacteria, quantity of NH_3-N and pH. However, the mechanism of how this salivary factors act in the caries activity test media was not yet known. Those unknown factors were investigated by the author employing the Snyder test and the Rickles test. The saliva of two groups of 25 female workers of a dentifrice Mfg. Co. aging from 16 to 23 were tested. The DF teeth rate (caries prevalence) of a group of 25 persons was 21.86±0.1881%, and the Snyder test and Rickles test of the saliva were positive (susceptible group), and in other (immune) group DF teeth rate was 6.00±0.1179%, and the caries activity tests were negative. At first, the number of lactobacilli, staphylococci and all anaerobic micro-organisms of the test specimen of the inoculated Snyder-and Rickles test media were counted at the decided interval of cultures. In the negative specimen of those two tests, lactobacilli were scarecely found. On the contrary, in the positive specimen, marked enumeration of lactobacilli were noted in the Snyder test media, and it was the interesting fact that the enumeration of lactobacilli was observed in the Rickles test media, although it was a little. The number of staphylococci increased slightly in the Snyder test media, but decreased in the Rickles test one. The number of anaerobic micro-organisms decreased rapidly in the media of both tests. As the results, it was conceived that lactobacilli were bacteriologically the chief factor which led the results of both tests positive. Secondly, the changes in the quantity of NH_3-N and the reducing sugar in the caries activity test media, to which the saliva were inoculated, were observed.Rise in quantity of NH_3-N was observed in both media when caries immune sailva were inoculated; on the contrary, the marked decrease of the reducing sugar in the media of both tests was observed when caries susceptible saliva were inoculated. Thirdly, the influence of the addition of vitamins, amino-acids and inorganic salts were investigated. No change was observed in the addition of those agents to the Snyder test media, however, the acceleration of the positive result of Rickles test (from 7 to 5 hours) in two hours was observed in case nicotinic acid or glutamic acid was added in this media. Fourthly, the addition of penicillin (antibiotic) and mercury chloride (disinfectant) inhibited the proliferation and fermentation of lactobacilli in both tests.
- 千葉大学の論文
- 1960-03-00