哺乳動物無菌飼育装置の研究
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概要
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In order to improve the apparatus for germ-free rearing of animals, the author studied on the methods of germ-free rearing historically and experimentally under the direction of Professor K. Tanikawa and Associate Professor J. Tanami. In our laboratory, a new compact type of rearing unit HMC-1 was constructed after Reyniers' system and Gustafsson's apparatus in 1954. Continuously the rearing unit HMC-2 was constructed with the useful advice given by Professor M. Miyakawa. A thermo-sterilizing plant and glass-wool filter are used for air sterilization. In order to eliminate humidity in the air a fin-coil type refrigerating desicator is used. The ventilation power of pump on the average is 60 1 per minute, and this quantity of air is sufficient for 2 rearing tanks and 1 operating tank. The rearing tank is constructed of steel 6 mm in thickness, the diameter of the tank is 60 cm and the length is 120cm. Entire surface of the tank is covered with asbestos in 3 cm thickness. This tank has 4 glove ports and 2 viewing ports, gloves are made of artificial rubber and are fixed on the glove ports of the tank with a special ring. The operating tank is almost equal to the rearing tank in its size and shape. This tank has a chamber for the operating trap provided under it. This trap is used for moving an animal into the tank. The sterilizing food clave is a small steel tank, 25 cm in diameter and 65 cm in length. This is used for removing food, water and the other heat steriliable articles into the rearing tank. The germicidal trap is an apparatus through which all objects placed into the rearing tank, which must not be sterilized with heat, must pass. The double Seitz-filter is an apparatus through which certain kinds of solution can be filtered and sterilized. The entire system can be sterilized at the same time, but in accordance with the demands of the experiment procedures, one part of the system, either the rearing tank or the operating tank, must be separately sterilized. Because of this reason a sterilizing pipe system is constructed for that purpose. The air temperature of the rearing tank is controlled by the air temperature regulator and the hot water tank provided under part of the tank, so there must be two electric regulating system for the rearing tank. All sorts of furnishings in the rearing tanks must be provided so as not to injure the ,rubber gloves during manipulation. The HMC-2 system is arranged to form a compact unit, so the system as a whole can be set in a chamber 3.6 m×7.2 m and can be used coveniently. It will be important to devise a compact system or simplified unit for germ-free rearing of animals so as to make rearing easy to perform in every laboratory wanting to do some experiments with germ-free animal. In the near future, a central system for germ-free animal production will be established in our country. At that time a simplified and compact system of germ-free rearing unit, such as the HMC-2, will become popular and convenient.
- 千葉大学の論文