Gancidinの試験管内生体内抗菌作用の研究(第2報)
スポンサーリンク
概要
- 論文の詳細を見る
Six fractions which had varying antibacterial and antitumor activity as well as physicochemical natures have been so far isolated from the crude material of gancidin. Two of those fractions were obtained as brick red and white crystals, and designated as gancidin A and gancidin W respectively. Later experiments suggested that gancidin A was a compound closely related with xanthomycin. Gancidin A was highly active against a wide range of test organisms including putrifactive and pathogenic bacteria. The most sensitive were gram positive cocci and gonorhoeae being inhibited as less as 1.0-0.0075 mcg per ml. However, the antibiotic exhibited only slight activity against mycobacterium. It was also strongly active against Ehrlich cancer cell in vitro, but the activity was only limited in vivo. So it is unlikely that gancidin A is the main antitumor principle present in the culture broth of streptomyces AAK-84. Gancidin W was only a slight antibacterial and antitumor substance, though it was also slightly toxic. The acquisition of resistance of several bacteria to gancidin A was tested. Only limited resistance was observed with M. aureus 209-p, M. albus, B. subtilis PCI-219, E. coli Fl, A. aerogenes and P. vulgaris after 20 subcultures on the gancidin A medium. Also there was observed no remarkable cross resistance between gancidin A and such known antibiotics as penicillin, streptomycin, chloromycetin, terramycin and violacetin. It was assumed that, as the results of those in vitro experiments, gancidin A had unprecedented (except xanthomycin) specific antibacterial activity. Gancidin A was readily absorbed and excreted into body fluid of experimental animals by subcutanous and intramuscular administration. However, according to its high toxicity, the antibiotic failed to protect mice from the infections of Diplococcus pneumoniae type 1 and Micrococcus pyogenes var. aureus Terajima.
- 千葉大学の論文