Golden Hamster(Cricetus auratus)の造精機転に就いて
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概要
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The author carried out investigations into the spermatogenesis of hamsters, using Heidenhain's Iron- hematoxylin stain, Benda's method and Morita's method of silver staining by tannic acid. The results are summarized as follows: 1) The present experiments have offered confirmation of Prof. Morita's assertion (in 1931) that various elements of the cytoplasm derive from the microsomes. 2) Both the mitochondria and Golgi apparatus of a spermatgonium are granular in form. 3) The mitochondria of a spermatocyte are found in such diversified forms as granular, chainlike, threadlike, rosarylike, looplike, etc. 4) As viewed from the standpoint of genesis, the centrosome can be traced to the same origin as the microsome. 5) Although the centosome is usually found inside of the idiosome, it sometimes is found outside of it. 6) It,sometimes is difficult to distinguish clearly between the "Cromatoider Nebenkorper," which is usually found near the idiosome and is larger than the microsome, and the large-sized mitochondria. 7) The idiosome of a spermatogonium is either looplike or half moon like, consists of the periphery that stains dark and the interior that stains light, and generally is smaller than its counterpart in a spermatocyte. 8) There are one or two idiosomes in a spermatocyte. They are found in as different forms, such as elliptic, half moon like, round, etc. as they show affinity for a vast variety of stains. 9) It has been observed that there is a granular or rod-shaped substance in the periphery of the idisome of a spermatocyte, which seems to be what was identified by Prof. Morita as the G. M. body. 10) The acrosome originates in what was identified by Prof. Morita as the vacuole with the granules. 11) What was termed "equatorial ring" by Prof. Morita as well as the head cap, so designated by Leblond and Clement (in 1952), has also been observed in the present experiments. Both of them consist of acrosome granules. 12) The "equatorial ring" is formed of a chain of granules.13) Metamorphosis of a cell after the formation of the "equatorial ring" is divided into four stages. 14) The first stage is characterized by the fact that a cell at this stage of development is pear-shaped. 15) At the first stage, the "equatorial ring" of a cell is observed in the anterior two thirds, and posterior one third, of the whole length of the nucleus. 16) The second stage is characterized by a sideways bend of the anterior portion of the nucleus which is almost perpendicular to its posterior portion with the "equatorial ring" as its base. 17) The third stage is characterized by a bend of the nucleus that takes place once again before it becomes sickle-shaped, the form specific to a sperm of this particular animal. 18) At the third stage, the "equatorial ring" is observed located diagonally, with its anterior end in the indented part of the head and its posterior end in the protuberant part of it. 19) Another trait of a cell at the third stage is the formation of membrane, consisting of the granules of the G.M. body, which is observed located posterior to the "equatorial ring." 20) The fourth stage is characterized by the development of the external part, which stains black, and the internal part, which stains light and is transparent, in the acrosome which grows to enwrap the sickle-shaped nucleus, also becoming sickle-shaped in the end. 21) The axial filament originates in the microsome, the granules of the axial filament split into the proximal granules and the distal granules, and the distal granules further split into what were termed nodulus anterior and nodulus posterior by Prof. Morita. 22) Deriving from the "equatorial ring," the tail sheath does not disappear but forms the side walls of the neck and middle piece of a spermatozoon. 23) What is called the neck of a spermatid extends from the posterior end of the head to nodulus anterior, covering the part surrounded by the tail sheath and the remnant of a thin cytoplasmic membrane on the side.24) There is, in the neck, the accessary body which is located close to the protuberant end of the head. 25) The middle piece, which is six times as long as the head, extends from nodulus anterior to the terminal ring. 26) The mitochondria granules of the middle piece, which have become threadlike, surround the axial filament spirally. 27) Constituting the distal portion of the middle piece, the tail consists of the main part and the end piece. 28) The main part, which is as long as or longer than the middle piece, consists of the axial filament and the thin cytoplasmic membrane that enwraps it. 29) The end piece, which consists of the naked axial filament, is very short.
- 千葉大学の論文
- 1956-05-28