Paenibacillus BRF-1 has biocontrol ability against Phialophora gregata disease and promotes soybean growth(Soil Biology)

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Gram-positive bacterium BRF-1 isolated from the rhizosphere of root-rotten diseased soybean plants shows strong antifungal activity on plates. In the present study, the ability of BRF-1 to control brown stem rot disease and to promote plant growth was examined using soybean plants grown in pots. Brown stem rot is a severe soil-borne disease of soybean that is caused by the pathogenic fungus Phialophora gregata. Eighteen-day-old soybean seedlings were grown in a mixture of soil (40mL) and BRF-1 suspension (10mL) and were dipped in a suspension of P. gregata (10^6c.f.u.mL^<-1>), and the severity of the disease was judged 62 days after the fungal inoculation. Inoculation with BRF-1 at 10^7 and 10^8c.f.u.mL^<-1> significantly lowered the severity of the disease. Soybean seeds incubated in 100mL of the BRF-1 suspension (2×10^6c.f.u.mL^<-1>) were sown in pots filled with sand, and plants were collected 7 days after emergence. The dry weight increase in shoots and roots and mineral absorption were significantly stimulated by the inoculation. These results suggest that BRF-1 might be an effective biocontrol agent to manage brown stem rot disease in soybean. The 16S rDNA sequence of BRF-1 showed high homology with sequences of Paenibacillus polymyxa strains (e.g. 99.7% identity with P. polymyxa strain WY110).
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