肝不全治療に向けた不死化ヒト肝細胞の樹立(<特集>がん疾患とアフェレシス)

概要

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For practical use of bio-artificial livers, the shortage of cell sources must be resolved. Immortalized human hepatocytes are expected to be among the new sources. In addition to telomerase activation by the introduction of human telomerase reverse transcriptase (hTERT), inactivation of the p16/RB pathway and/or p53 by E6/E7 of human papillomavirus type 16 (HPV16) has been shown to be useful for efficient immortalization of several human cell types. We have established two human immortalized hepatocyte cell lines by the introduction of HPV16 E6/E7 and hTERT, then named them HHE6 E7 T-1 and HHE6 E7 T-2. Those cells proliferated in culture beyond 200 population doublings (PDs). Albumin synthesis and expression of liver-enriched genes were confirmed. Karyotype analysis showed that HHE6 E7 T-1 cells remained near diploid at 150PDs. Intrasplenic transplantation of HHE6 E7 T-1 cells significantly improved the survival of acetaminophen-induced acute liver failure SCID mice.
日本アフェレシス学会の論文
2009-05-31