Functional biosynthesis of an allophycocyan beta subunit in Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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概要

• 論文の詳細を見る

• Allophycocyanin is a phycobiliprotein with various biological and pharmacological properties. An expression vector was constructed using CpeS as the bilin lyase for the allophycocyanin β subunit, resulting in overexpression of a fluorescent allophycocyanin β-subunit in Escherichia coll. A high-density cell culture was developed using a continuous feeding strategy. After 16h of culture, the dry cell density reached 21.4g l^<-1>, the expression of the allophycocyanin β-subunit was 0.86g l^<-1> broth, and the relative chromoprotein yield was 81.4%. The recombinant protein showed spectral features similar to native allophycocyanin, which provide an efficient methodology for large-scale production of this valuable fluorescent protein.

• 社団法人日本生物工学会の論文

著者

• QIN Song

  Institute of Oceanology, Chinese Academy of Sciences

• Feng Yang

  Center For Biotechnology And Bioengineering China University Of Petroleum

• Qin Song

  Institute Of Oceanology Chinese Academy Of Sciences

• Ge Baosheng

  Center for Biotechnology and Bioengineering, China University of Petroleum

• Sun Haixiang

  College of Chemistry and Chemical Engineering, China University of Petroleum

• Yang Jinying

  Center for Biotechnology and Bioengineering, China University of Petroleum

• Ge Baosheng

  Center For Biotechnology And Bioengineering China University Of Petroleum:jiangsu Key Laboratory Of

• Yang Jinying

  Center For Biotechnology And Bioengineering China University Of Petroleum

• Sun Haixiang

  College Of Chemistry And Chemical Engineering China University Of Petroleum

関連論文

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• Chandrananimycins A~C : Production of Novel Anticancer Antibiotics from a Marine Actinomadura sp. Isolate M048 by Variation of Medium Composition and Growth Conditions
• Functional biosynthesis of an allophycocyan beta subunit in Escherichia coli(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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