Electron Microscopic Study of In Vitro Vibrio vulnificus Infection to Tissue Culture Cells and Peripheral Blood Cells
スポンサーリンク
概要
- 論文の詳細を見る
Vibrio vulnificus is the most pathogenic species among the genus Vibrio, and such a fatal V. vulnificus infection case occurred in Niigata in 2003. In this study, we investigated the in vitro characteristics of V. vulnificus infection to tissue culture cells (HEp-2, HeLa, and HCT-8) and human peripheral blood cells by scanning and transmission electron microscopy. V. vulnificus damaged those cells, especially HeLa cells. Moreover, V. vulnificus adhered to cells possessed unique pearl-like structure, unlike V. cholerae O1 or O139 and V. parahaemolyticus. When human peripheral blood cells were infected with V. vulnificus, leukocytes and platelets were markedly adhered with V. vulnificus, and platelet was most seriously damaged. Separation of the outer and inner nuclear membranes was observed in some blood cells such as leukocytes. Pilus-mediated V. vulnificus adherence to blood cells was also observed. Invasion (or uptake) of V. vulnificus into blood cells was not obvious, in contrast to methicillin-resistant Staphylococcus aureus. The data suggest that V. vulnificus may use unique appendages for infection, and may damage blood cells such as platelets and leukocytes by producing toxins rather than invading cells, and prevent phagocytosis by bacterium-engulfing cells by toxins.
- 新潟大学の論文
著者
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YAMAMOTO Tatsuo
Division of Bacteriology, Niigata University Graduate School of Medical and Dental Sciences
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Takano Tomomi
Niigata Univ. Graduate School Of Medical And Dental Sci. Niigata Jpn
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Yamamoto Tatsuo
Niigata Univ. Graduate School Of Medical And Dental Sci. Niigata Jpn
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HIGUCHI Wataru
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niiga
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TAKANO Tomomi
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niiga
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BARANOVICH Tatiana
Division of Bacteriology, Department of Infectious Disease Control and International Medicine, Niiga
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IAWKURA Nobuhiro
Division of Bacteriology, Department of Infectious Disease Control and International Medicine
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NAKAYAMA Teruko
Division of Bacteriology, Department of Infectious Disease Control and International Medicine
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SHIBUYA Michiko
Division of Bacteriology, Department of Infectious Disease Control and International Medicine
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ITOH Masaaki
Division of Dermatology, Department of Cellular Function, Niigata University Graduate School of Medi
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Higuchi Wataru
Niigata Univ. Graduate School Of Medical And Dental Sci. Niigata Jpn
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Yamamoto Tatsuo
Division Of Bacteriology Department Of Infectious Disease Control And International Medicine Niigata
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Takano Tomomi
Division Of Bacteriology Department Of Infectious Disease Control And International Medicine Niigata
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Yamamoto T
Division Of Bacteriology Department Of Infectious Disease Control And International Medicine
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Higuchi Wataru
Division Of Bacteriology Department Of Infectious Disease Control And International Medicine Niigata
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Itoh Masaaki
Division Of Dermatology Department Of Cellular Function Niigata University Graduate School Of Medica
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Shibuya Michiko
Division Of Bacteriology Department Of Infectious Disease Control And International Medicine:divisio
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Takashi Nakayama
Division Of Bacteriology Department Of Infectious Disease Control And International Medicine
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Iwakura Nobuhiro
Division Of Bacteriology Department Of Infectious Disease Control And International Medicine
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Baranovich Tatiana
Division Of Bacteriology Department Of Infectious Disease Control And International Medicine Niigata
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Yamamoto T
Division Of Bacteriology Department Of Infectious Disease Control And International Medicine Niigata
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Takano Tp
Division of Bacteriology, Department of Infectious Disease Control and International Medicine
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