Establishment of a Method for Analyzing Translesion DNA Synthesis across a Single Bulky Adduct in Human Cells

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概要

• 論文の詳細を見る

• Translesion DNA synthesis (TLS) is a tolerance pathway of replication block caused by DNA lesions. To measure the efficiency and fidelity of TLS in human cells, we established a shuttle vector assay by modifying of a bacterial TLS assay system. The assay consists of transfection of DNA repair-deficient human cells with a plasmid possessing a single DNA adduct, and transformation of indicator bacteria with plasmids extracted from the cells. We show that plasmid replication was suppressed to 1/9 by a single aminobiphenyl-dG adduct, and the mutant frequency of TLS-operated plasmids was 0.31, of which the major mutation (78%) was G to T transversion. The results demonstrate that this assay is applicable in practice for investigating TLS in human cells.

• 日本環境変異原学会の論文
• 2009-02-20

著者

• Takamura-enya Takeji

  Department Of Applied Chemistry Kanagawa Institute Of Technology

• Sawai Tomoko

  Environmental Genetics Laboratory, Frontier Science Innovation Center and Department of Biology, Gra

• Kawanishi Masanobu

  Environmental Genetics Laboratory, Frontier Science Innovation Center and Department of Biology, Gra

• Yagi Takashi

  Environmental Genetics Laboratory, Frontier Science Innovation Center and Department of Biology, Gra

• Sawai Tomoko

  Environmental Genetics Laboratory Frontier Science Innovation Center And Department Of Biology Gradu

• Yagi Takashi

  Environmental Genetics Laboratory Frontier Science Innovation Center And Graduate School Of Science

• Kawanishi Masanobu

  Environmental Genetics Laboratory Frontier Science Innovation Center And Department Of Biology Gradu

• Sawai Tomoko

  Environmental Genetics Laboratory Frontier Science Innovation Center And Department Of Biology Gradu

• Yagi Takashi

  Environmental Genetics Laboratory Frontier Science Innovation Center And Department Of Biology Gradu

関連論文

• Establishment of a Method for Analyzing Translesion DNA Synthesis across a Single Bulky Adduct in Human Cells
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