Application of Real Time PCR for Diagnosis of Swine Dysentery(Public Health)

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概要

• 論文の詳細を見る

• Evaluation of a genetic diagnostic technique using real time PCR of Swine Dysentery (SD) was performed using nox primers. Culture, ordinary PCR and real time PCR were compared in this experiment. Sixty-seven specimens from pigs with clinical signs of SD brought to a slaughterhouse in Shibaura, Tokyo, were used. B. hyodysenteriae was isolated from 49 of the pigs, was detected by ordinary PCR in 49 of the pigs and was detected by real time PCR in 54 of the pigs. Furthermore, we were able to determine the numbers of B. hyodysenteriae cells in all positive specimens by real time PCR. The rapid diagnostic technique established in this experiment was useful for detection of B. hyodysenteriae because it was more effective than ordinary PCR and culture.

• 社団法人日本獣医学会の論文
• 2009-03-25

著者

• Sadamasu Kenji

  Department Of Microbiology Tokyo Metropolitan Research Laboratory Of Public Health

• Sadamasu Kenji

  Department Of Microbiology Tokyo Metropolitan Institute Of Public Health

• AKASE Satoru

  Health and Safety Division, Bureau of Social Welfare and Public Health, Tokyo Metropolitan Governmen

• UCHITANI Yumi

  Department of Microbiology, Tokyo Metropolitan Institute of Public Health

• SOHMURA Yoshiko

  Johnanjima Branch Office, Animal Care and Consultation Center, Bureau of Social Welfare and Public H

• TATSUTA Keikichi

  Shibaura Meat and Sanitary Inspection Station, Bureau of Social Welfare and Public Health, Tokyo Met

• ADACHI Yoshikazu

  College of Agriculture, Ibaraki University

• Adachi Yoshikazu

  College Of Agriculture Ibaraki University:united Graduate School Of Agricultural Science Tokyo Unive

• Sohmura Yoshiko

  Johnanjima Branch Office Animal Care And Consultation Center Bureau Of Social Welfare And Public Hea

• Tatsuta Keikichi

  Shibaura Meat And Sanitary Inspection Station Bureau Of Social Welfare And Public Health Tokyo Metro

• Uchitani Yumi

  Department Of Microbiology Tokyo Metropolitan Institute Of Public Health

• Akase Satoru

  Health And Safety Division Bureau Of Social Welfare And Public Health Tokyo Metropolitan Government:

• Adachi Yoshikazu

  College Of Agriculture Ibaraki University

関連論文

• Application of Real Time PCR for Diagnosis of Swine Dysentery(Public Health)
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