Possible involvement of proteinaceous and non-proteinaceous trehalase inhibitors in the regulation of hemolymph trehalose concentration in Bombyx mori
スポンサーリンク
概要
- 論文の詳細を見る
The midgut of the silkworm Bombyx mori contains two types of trehalase: soluble and membrane-bound. The trehalose concentration in larval hemolymph remains substantially high through the feeding period of the last (fifth) larval instar, despite appreciable trehalase activity through this period. We found that B. mori larval hemolymph contains two types of trehalase inhibitor, a proteinaceous inhibitor (inhibitor-P) and an inhibitor that is extractable with methanol and ethanol (inhibitor-M). Both inhibit the two types of trehalase, but with different sensitivities. Inhibitor-P inhibited soluble trehalase (ST) more effectively than membrane-bound trehalase (MT), while inhibitor-M inhibited MT slightly more than ST. The inhibitory activity of inhibitor-M was high during the early to middle feeding period, followed by a sudden decrease during the late feeding period. Inhibitor-P activity increased throughout the feeding period, and markedly decreased 2 days after gut purge. Thus, the two inhibitors exhibited stage-specific changes in the hemolymph, indicating coordinated inhibitory activity. Therefore, trehalose concentration regulation in the hemolymph appears to be complex, consisting of at least two inhibitors and two types of trehalase.
- 日本応用動物昆虫学会の論文
- 2009-02-25
著者
-
Iwami Masafumi
Division Of Life Science Graduate School Of Natural Science And Technology And Department Of Biology
-
Sakurai Sho
Division Of Life Science Graduate School Of Natural Science And Technology Kanazawa University:depar
-
Singtripop Tippawan
Department Of Biology Faculty Of Science Chiang Mai University
-
TATUN Nujira
Department of Biology, Faculty of Science, Chiang Mai University
-
OSUGI Shingo
Division of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University
-
NACHIANGMAI Siriluck
Department of Biology, Faculty of Science, Chiang Mai University
-
Osugi Shingo
Division Of Life Sciences Graduate School Of Natural Science And Technology Kanazawa University
-
Tatun Nujira
Department Of Biology Faculty Of Science Chiang Mai University
-
Nachiangmai Siriluck
Department Of Biology Faculty Of Science Chiang Mai University
関連論文
- Larval Growth and Diapause in a Tropical Moth, Omphisa fuscidentalis Hampson(Physiology)
- Developmental Profile of Annexin IX and its Possible Role in Programmed Cell Death of the Bombyx mori Anterior Silk Gland(Developmental Biology)
- AN EXHAUSTIVE STUDY ON THE ECDYSONE-INDUCIBLE GENES IN THE SILKWORM BRAIN DURING METAMORPHOSIS(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- EXPRESSION PROFILES OF ECDYSONE RECEPTOR ISOFORMS AND EARLY GENES ASSOCIATE THE PUPAL COMMITMENT IN THE WING DISCS OF THE SILKWORM, BOMBYX MORI(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- ECDYSTEROID CONTROLS PUPAL COMMITMENT AND CELL CYCLE IN THE WING DISCS OF THE SILKWORM, BOMBYX MORI(Endocrinology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- HORMONAL CONTROL OF THE CELL CYCLE IN THE WING DISCS OF THE SILKWORM, BOMBYX MORI
- Solubilization of the ecdysone binding protein from anterior silk gland cell membranes of the silkworm, Bombyx mori
- DEVELOPMENTAL CHANGES OF PROTHORACIC GLAND ACTIVITY AND ITS RESPONSIVENESS TO PTTH IN BOMBYX MORI(Endocrinology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- ANNEXIN IX PARTICIPATES IN THE DETERMINATION OF TIMING OF PCD(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Ecdysteroid-inducible genes in the programmed cell death of anterior silk gland of silkworm, Bombyx mori(Endocrinology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- HORMONAL CONTROL OF DEATH COMMITMENT IN THE ANTERIOR SILK GLAND OF THE SILKWORM, BOMBYX MORI(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- EXPRESSION PROFILES OF EARLY AND EARLY-LATE GENES IN THE STEROID-INDUCED PROGRAMMED CELL DEATH IN THE ANTERIOR SILK GLANDS OF BOMBYX MORI(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- EXPRESSION PROFILES OF EARLY AND EARLY-LATE GENES IN THE STEROID-INDUCED PROGRAMMED CELL DEATH IN THE ANTERIOR SILK GLANDS OF BOMBYX MORI(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- EARLY AND EARLY-LATE GENE EXPRESSIONS IN THE ANTERIOR SILK GLAND OF THE SILKWORM, BOMBYX MORI DURING PROGRAMMED CELL DEATH(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- ENDOCRINE MECHANISMS TO MAINTAIN THE LARVAL DIAPAUSE IN THE BAMBOO BORER, OMPHISA FUSCIDENTALIS(Endocrinology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- HORMONAL MECHANISMS UNDERLYING DIAPAUSE BREAK BY JUVENILE HORMONE IN THE BAMBOO BORER, OMPHISA FUSCIDENTALIS(Endocrinology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- A Rapid Increase in cAMP in Response to 20-Hydroxyecdysone in the Anterior Silk Glands of the Silkworm, Bombyx mori(Endocrinology)
- PRESENCE OF MEMBRANE ECDYSONE RECEPTOR IN THE ANTERIOR SILK GLAND OF THE SILKWORM, BOMBYX MORI(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- NON-STEROID SIGNAL CASCADE IN CELL DEATH OF THE ANTERIOR SILK GLANDS OF BOMBYX MORI(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- 20-HYDROXYECDYSON SIGNAL CASCADE IN PROGRAMMED CELL DEATH OF THE ANTERIOR SILK GLANDS OF BOMBYX MORI(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- VARIATION OF PROGRAMMED CELL DEATH IN THE ANTERIOR SILK GLANDS OF BOMBYX MORI(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- TWO TRANSCRIPTION ELEMENTS OF THE BOMBYXIN F1 GENE REGULATE THE BRAIN CELL-SPECIFIC EXPRESSION(Genetics)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- CHARACTERIZATION OF ECDYSONE MEMBRANE RECEPTOR REQUIRED FOR THE PROGRAMMED CELL DEATH IN BOMBYX MORI(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Analysis of Pupal commitment in the wing disc of the silkworm, Bombyx mori(Endocrinology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Transcription element responsible for the brain cell-specific expression of the Bombyxin gene that encodes an insect insulin-related peptide
- Role of ecdysteroids in the dynamics of insect haemolymph sugar
- PROMOTER ANALYSIS SYSTEM OF INSECT BEUROPEPTIDE GENES USING BACULOVIRUS(Genetics)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Possible involvement of proteinaceous and non-proteinaceous trehalase inhibitors in the regulation of hemolymph trehalose concentration in Bombyx mori
- Intensity of larval diapause in the bamboo borer, Omphisa fuscidentalis