The Biosynthesis of Kasugamycin, an Antibiotic against Rice Blast Disease, with particular Reference to the involvement of rpoZ, a Gene Encoding RNA Polymerase Omega Subunit

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概要

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• Biosynthetic genes of kasugamycin (KSM), an aminoglycoside antibiotic produced by Streptomyces kasugaensis, forms a cluster within the chromosome. A cloned 22.4-kb cluster region contains almost allthe enzyme-coding genes required for KSM biosynthesis together with kac338, a gene for KSM acetyltransferase, and kasKLM, a setof genes encoding an ABC transporter, both of which participate in KSM self-resistance as well as kasT encoding a KSM-synthesis-specific transcriptional activator of the biosynthetic genes. Furthermore, rpoZ, encoding a 90-amino acid omega (ω) subunit of RNA polymerase (RNAP), is required for the simultaneous production of KSM and aerial mycelium, and rpoZ-disrupted wild-type S. kasugaensis produces neither KSM nor aerial mycelia. Transcriptional analysis of the biosynthetic genes and forcible expression of kasT in the mutant revealed that the presence of rpoZ, which resultsin formation of RNAP carrying the co subunit, facilitates initiation of kasT transcription and is thus crucial for KSM production.

• 秋田大学の論文

著者

• Kasuga Kano

  Akita Prefectural University

• Kojima Ikuo

  Akita Prefectural University

• Kobayashi Masayuki

  Akita Prefectural University

• Tsuchiya Kayako

  Showa Pharmaceutical University

• Ikeno Souici

  Showa Pharmaceutical University

• Kobayashi Masayuki

  Morinaga Milk Branch Research Institute Of Innovative Technology For The Earth C/o Biochemical Resea

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