The Role of Calreticulin Transacetylase in the Activation of Human Platelet Nitrite Reductase by Polyphenolic Acetates(Biochemistry)

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概要

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• Our earlier investigations demonstrated the remarkable activation of cytochrome P-450 reductase and nitric oxide synthase by 7,8-diacetoxy-4-methylcoumarin, a model polyphenolic acetate by way of acetylation, catalyzed by the Calreticulin. Protein acetyltransferase action of Calreticulin was hence termed Calreticulin transacetylase (CRTAase). Nitric oxide synthase and nitrite reductase are now considered as parts of nitric oxide cycle. The activation of platelets nitric oxide synthase by 7,8-diacetoxy-4-methylcoumarin has already been demonstrated by us. Also, there are reports that certain proteins such as cytochrome P-450 reductase and cytochrome P-450 are endowed with the nitrite reductase activity in mammalian cells. Keeping these facts in view, we turned our attention to probe whether 7,8-diacetoxy-4-methylcoumarin could alter the levels of nitric oxide independent of the action of nitric oxide synthase in the human platelets model. The incubation of 7,8-diacetoxy-4-methylcoumarin and nitrite with platelets caused significant elevation of nitric oxide and cyclic guanosine monophosphate levels possibly due to the activation of nitrite reductase. Several polyphenolic acetates were similarly found to activate the nitrite reductase in tune with their affinities as substrate to CRTAase. N-ω-Nitro-L-arginine methyl ester, the inhibitor of nitric oxide synthase, failed to reverse such an effect of 7,8-diacetoxy-4-methylcoumarin. Clotrimazole which is known to be an inhibitor of nitrite reductase, effectively abolished the 7,8-diacetoxy-4-methylcoumarin mediated enhancement of nitric oxide levels in platelets as well as the nitric oxide mediated effects; such as cyclic guanosine monophosphate levels as well as adenosine diphospate induced platelets aggregation due to nitrite.

• 公益社団法人日本薬学会の論文
• 2009-02-01

著者

• Raj Hanumantharao

  Department Of Biochemistry V.p. Chest Institute University Of Delhi

• Majumder Syamantak

  Aukbc Research Center Mit Campus Anna University

• Tyagi Yogesh

  School Of Basic And Applied Sciences Guru Gobind Singh Indraprastha University

• KUMAR Ajit

  Department of Biochemistry and Microbiology, V.P. Chest Institute, University of Delhi

• PARMAR Virinder

  Department of Chemistry, University of Delhi

• ARORA Shvetambri

  Department of Biochemistry, V.P. Chest Institute, University of Delhi

• SALUJA Daman

  Dr. B.R. Ambedkar Center of Biomedical Research, University of Delhi

• CHATTERJEE Suvro

  AUKBC Research Center, MIT Campus Anna University

• SASO Luciano

  Department of Human Physiology and Pharmacology "Vittorio Erspamer" Sapienza University of Rome

• PRASAD Ashok

  Department of Chemistry, Bioorganic Laboratory, University of Delhi

• Prasad Ashok

  Department Of Chemistry Bioorganic Laboratory University Of Delhi

• Kumar Ajit

  Department Of Biochemistry V.p. Chest Institute University Of Delhi

• Saluja Daman

  Dr. B.r. Ambedkar Center Of Biomedical Research University Of Delhi

• Saso Luciano

  Department Of Human Physiology And Pharmacology "vittorio Erspamer" Sapienza University Of

• Parmar Virinder

  Department Of Chemistry Bioorganic Laboratory University Of Delhi

• Chatterjee Suvro

  Aukbc Research Center Mit Campus Anna University

• Arora Shvetambri

  Department Of Biochemistry V.p. Chest Institute University Of Delhi

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• The Role of Calreticulin Transacetylase in the Activation of Human Platelet Nitrite Reductase by Polyphenolic Acetates(Biochemistry)
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