Continuous hydrogen production from glucose by using extreme thermophilic anaerobic microflora(ENVIRONMENTAL BIOTECHNOLOGY)

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Continuous hydrogen production from glucose at 75℃ was examined at HRTs of 0.67, 1,2, and 3d by using extreme thermophilic microflora, which consisted of several subspecies of Caldanaerobacter subterraneus. The maximum hydrogen yield of 3.32mol-H_2/mol-glucose degraded was obtained at the highest HRT, 3d. Both the maximum volumetric and specific rates of hydrogen production (1.22L-H_2/L/d and 4.15L-H_2/g-VSS/d, respectively) were obtained at the lowest HRT, 0.67d. A 16S rDNA analysis showed that the community structure of the microflora did not change at any of the HRTs.