チャ品種'さやまかおり'の組織培養におけるカルス誘導

概要

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No reproducible and efficient transformation system has been developed, despite many attempts made to obtain transformed plants in tea (Camellia sinensis). This is because no efficient and stable tissue culture system for tea has been developed yet. In order to develop such a system, it is essential analyze the culture conditions. In this examination, stem segments including epidermal layers of Camellia sinensis cv. 'Sayamakaori' used as the explants to induce the callus producing shoots. The explants cultured on the 9 combination of 3-Indolebutyric acid (IBA) and 6-Benzylaminopurine (BA) added in the culture medium. Calli were obtained on the several media containing IBA and BA.
沼津工業高等専門学校の論文
2008-01-31