大腸菌WP3101P〜WP3106P株を用いた突然変異誘発スペクトルの解析に関する共同研究 : 前培養培地の影響

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Lac^+ reversion assay with the E. coli strains WP3101P-WP3106P, derivatives of WP2uvrA/pKM101, have been used to determine mutational specificity of mutagens. A minimal glucose medium supplemented with tryptophan (MGT) is used as a preculture medium in the Lac^+ reversion assay. Nutrient broth (NB), on the other hand, is usually used for Trp^+ reversion assay with the strain WP2uvrA/pKM101. To improve the test procedure using a common preculture medium, we investigated the sensitivity of tester strains WP3101P-WP3106P against 11 kinds of mutagens when they were precultured in NB or MGT medium. In the Lac^+ reversion assay, cells grown in MGT were as sensitive to MNNG, ENNG, EMS, and N^4-aminocytidine as those grown in NB. However, mutagenicities of MMS, captan, 5-diazouracil, and Na-azide were scarcely detected with NB-cultured cells. In contrast, cells grown in MGT were less sensitive to several mutagens in the Trp^+ reversion assay than cells grown in NB. Therefore the use of a common preculture medium is not recommended for the Lac^+ and Trp^+ reversion assays.
2003-04-30