Effect of hydroalcoholic extracts of Tylophora indica leaves in ischemia-reperfusion induced myocardial damage using isolated rat heart

概要

論文の詳細を見る
The present study was carried out to determine the effect of hydroalcoholic extract of Tylophora indica (HETI) on experimentally induced myocardial infarction (MI) in rats. Albino rats were treated with HETI at two different doses of 100mg/kg, (HETI-100), 200mg/kg (HETI-200) and propranolol 10mg/kg (PRO-10) for 30 days orally. The hearts were excised and mounted on modified langendorff setup and subjected to 15 min global no flow ischemia and reperfused for 15 min for inducing ischemia-reperfusion injury (IRI). Pretreatment of animals with PRO-10 and HETI-200 provided significant protection to myocardium from IRI damage as indicated by significant decrease in LDH and CK-MB activities in perfusate and an increase in activities of these enzymes in heart tissue homogenate (HTH). Similarly, the recovery (%) in developed tension and heart rate were significantly high in HETI-200 and PRO-10 during post-ischemia when compared to control. Moreover, HETI-200 significantly increases endogenous antioxidants (SOD and catalase) activities when compared to IRI control. The protection offered by HETI could be attributed to the presence of flavanoids which shows antioxidant effect by either inhibiting the release of OFR or enhancing the synthesis of endogenous antioxidants such as SOD and catalase in IRI induced cardiotoxicity. These biochemical findings were further confirmed by histological investigations.
2008-11-29