Cultured osteoblast synthesize nitric oxide in response to cytokines and lipopolysaccharide.

スポンサーリンク

概要

• 論文の詳細を見る

• Nitric oxide (NO) is generated from L-arginine by NO synthase. NO has been reported to be produced by a variety of cell types such as vascular endothelial cells, macrophages, neutrophils and articular chondrocytes. A recent report demonstrated that NO inhibits osteoclast (OC) function and, in this way, is critically associated with bone metabolism. In the present study we have studied NO synthesis by osteoblasts (OBs). OB cell line, MC3T3-E1, was cultured with the various cytokines for 72 hrs. Nitrite, a stable endproduct of cell-generated NO, in the culture supernatant was then determined by using a spectrophotometric method based on Griess reaction. IL-1 alpha increased nitrite release in a dose-dependent fashion and a significant enhancement (p < 0.01) was attained at 10 U/ml. OBs released 14.2 nmol/4.0 x 10(4) cells of nitrite after 72 hrs stimulation by 100 U/ml IL-1 alpha. In contrast IL-1 beta, TNF-alpha and INF-gamma failed to affect NO synthesis by MC3T3-E1. The results suggest that OBs produce NO in response to IL-1 alpha and OB-induced NO may play a role in OB-OC interaction in the inflammatory process.

• 神戸大学の論文

著者

• Mizuno K

  Department Of Biochemistry Nagoya City University Medical School

• Matsubara T

  Department Of Chemical Biology Osaka City University Medical School

• Ake Y

  Department Of Orthopaedic Surgery Kobe University School Of Medicine

• Saegusa Y

  Department Of Orthopedic Surgery Kobe University School Of Medicine

• Mizuno K

  Department Of Orthopedic Surgery Kobe University School Of Medicine

• Matsubara T

  Department Of Orthopedic Surgery Kobe University School Of Medicine

関連論文

• The Effect of Lipid Peroxide on Osteoblasts and Vascular Endothelial Cells : The Possible Role of Ischemia-Reperfusion in the Progression of Avascular Necrosis of the Femoral Head
• Safety Evaluation and Drug Development based on Biological Fate of Drugs : Efforts Made to Overcome Drug Interaction in Drug Development
• Cultured osteoblast synthesize nitric oxide in response to cytokines and lipopolysaccharide.
• The osteogenic potential of fracture hematoma and its mechanism on bone formation--through fracture hematoma culture and transplantation of freeze-dried hematoma.
• A comparison of the bone mineral density of the vertebral bodies and the hip in elderly females with hip fractures. Assessment using dual photon absorptiometry.
• The relation between cubital tunnel syndrome and the elbow alignment.

スポンサーリンク