Protein-Energy Malnutrition Modifies the Production of Interleukin-10 in Response to Lipopolysaccharide (LPS) in a Murine Model

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概要

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• Malnutrition modifies resistance to infection by impairing a number of physiological processes including hematopoesis and the immune response. In this study, we examined the production of Interleukin-4 (IL-4) and IL-10 in response to lipopolysaccharide (LPS) and also evaluated the cellularity of the blood, bone marrow, and spleen in a mouse model of protein-energy malnutrition. Two-month-old male Swiss mice were subjected to protein-energy malnutrition (PEM) with a low-protein diet (4%) as compared to the control diet (20%). When the experimental group lost approximately 20% of their original body weight, the animals from both groups received 1.25μg of LPS intravenously. The cells in the blood, bone marrow, and spleen were counted, and circulating levels of IL-4 and IL-10 were evaluated in animals stimulated with LPS. Cells from the spleen, bone marrow, and peritoneal cavity of non-inoculated animals were collected for culture to evaluate the production of IL-4 and IL-10 after stimulating these cells with 1.25μg of LPS in vitro. Malnourished animals presented leucopenia and a severe reduction in bone marrow, spleen, and peritoneal cavity cellularity before and after stimulus with LPS. The circulating levels of IL-10 were increased in malnourished animals inoculated with LPS when compared to control animals, although the levels of IL-4 did not differ. In cells cultured with LPS, we observed high levels of IL-10 in the bone marrow cells of malnourished animals. These findings suggest that malnourished mice present a deficient immune response to LPS. These alterations may be partly responsible for the immunodeficiency observed in these malnourished mice.

• 日本ビタミン学会の論文

著者

• Rogero Marcelo

  Department Of Food And Experimental Nutrition School Of Pharmaceutical Sciences University Of Sao Pa

• FOCK Ricardo

  Experimental Hematology Laboratory, Department of Clinical and Toxicological Analyses, School of Pha

• VINOLO Marco

  Experimental Hematology Laboratory, Department of Clinical and Toxicological Analyses, School of Pha

• CRISMA Amanda

  Experimental Hematology Laboratory, Department of Clinical and Toxicological Analyses, School of Pha

• NAKAJIMA Karina

  Experimental Hematology Laboratory, Department of Clinical and Toxicological Analyses, School of Pha

• BORELLI Primavera

  Experimental Hematology Laboratory, Department of Clinical and Toxicological Analyses, School of Pha

• Fock Ricardo

  Experimental Hematology Laboratory Department Of Clinical And Toxicological Analyses School Of Pharm

• Vinolo Marco

  Experimental Hematology Laboratory Department Of Clinical And Toxicological Analyses School Of Pharm

• Crisma Amanda

  Experimental Hematology Laboratory Department Of Clinical And Toxicological Analyses School Of Pharm

• Nakajima Karina

  Experimental Hematology Laboratory Department Of Clinical And Toxicological Analyses School Of Pharm

• Borelli Primavera

  Experimental Hematology Laboratory Department Of Clinical And Toxicological Analyses School Of Pharm

関連論文

• Protein-Energy Malnutrition Modifies the Production of Interleukin-10 in Response to Lipopolysaccharide (LPS) in a Murine Model

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