P-30 Isolation resveratrol from peanut root (Arachis hypogaea L.) and establishment of method for quantitative analysis

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A phytoalexin, trans-resveratrol (3,4',5-trihydroxystilbene) was isolated from the ethylacetate soluble fraction from peanut root (Arachis hypogaea L.) by the repeated silica gel column chromatography. It was elucidated by the extensive spectroscopic analysis including MS, ^1H- and ^<13>C-NMR data. Optimum conditions for resveratrol quantitative analysis were determinded. The best conditon for solid phase, mobile phase and UV detection were Zorbax SB-C18 (4.6×150mm, 3.5μm), Water: MeCN (1%HOAc) and 254nm respectively. The equation of the regression coefficients for trans-resveratrol standard was y=0.001X+0.0021 (r^2=0.9994). Under these conditions, the value of RSD from peak area and retention time was 0.40%, 0.03%. The values of CV and accuracy in intra-day according to concentration of standard were 0.70%, 102.7%. trans-resveratrol content from peanut root was 1.7mg/g. These results might give useful information for recycling of peanut roots, since the isolated compounds have been known to have many beneficial activities such as antioxidative, anticancer and antimutagenic effects.
日本作物学会の論文
2008-03-27