Effects of an AMP-Activated Protein Kinase Inhibitor, Compound C, on Adipogenic Differentiation of 3T3-L1 Cells(Molecular and Cell Biology)
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概要
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The role of AMP-activated protein kinase (AMPK) in adipocyte differentiation is not completely understood. Here we reported that an AMPK inhibitor, compound C, significantly inhibited adipogenic differentiation of 3T3-L1 cells in a dose dependent manner, and this inhibitory effect was primarily effective in the initial stage of differentiation. Compound C prevented the mitotic clonal expansion (MCE) of preadipocytes, probably by inhibiting expression of CCAAT/enhancer-binding protein (C/EBP) β and δ, and subsequently blocked the expression of C/EBP α and peroxisome proliferator-activated receptor (PPAR) γ and transcriptional activation of genes that produce the adipocyte phenotype. AMPK activity was also suppressed by compound C treatment during the early phase of adipogenic differentiation, which indicated that suppressed activation of AMPK by compound C may inhibit the MCE process of preadipocytes. Our results suggest that compound C might serve as a useful molecule in both basic and clinical research on adipogenesis and as a potential lead compound for the treatment of obesity.
- 公益社団法人日本薬学会の論文
- 2008-09-01
著者
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Gao Ye
Guangzhou Institute Of Biomedicine And Health Chinese Academy Of Sciences International Business Inc
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ZHOU Yi
Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, International Business I
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XU Aimin
Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, International Business I
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WU Donghai
Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, International Business I
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Xu Aimin
Dep. Of Medicine And Pharmacology Univ. Of Hong Kong
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Wu Donghai
Dep. Of Biomedicine And Biotechnology Univ. Of Sci. And Technol. Of China
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Zhou Yi
Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, International Business Incubator