Positron Emission Tomography (PET)イメージング法による小動物用インビボ局所脳酸素代謝率測定法の開発に関する研究
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The cerebral metabolic rate for oxygen (CMRO_2) and cerebral oxygen extraction fraction (OEF) are two fundamental parameters used to characterize the pathophysiologic status of cerebral tissues. Although the O-15-labeled gas inhalation method is used to measure these parameters in clinical studies, applying this method to small animals requires many intensive procedures. Thus the development of a new method to measure CMRO_2 and OEF in small animals is of interest. This study aimed to develop a method to assess CMRO_2 and OEF using intravenously injectable oxygen (injectable ^<15>O-O_2) and Positron Emission Tomography (PET) for small animals such as rats. Injectable ^<15>O-O_2 was obtained after ^<15>O-O_2 gas circulation into an artificial lung. The OEF in normal rats was calculated using the same equation as that used for the bolus inhalation in the ^<15>O-O_2 gas method. The obtained value of 0.54±0.11 of OEF was similar to the value determined from the arterial-venous difference in the oxygen concentration. Furthermore, we evaluated the usefulness of the injectable ^<15>O-O_2 system in rats occluded in the right middle cerebral artery. A decrease in cerebral blood flow (CBF) and compensatory increase in OEF were observed 1h after occlusion. In contrast, a marked decrease in CBF and CMRO_2 and a collapse of the compensatory OEF mechanism were found 24h after occlusion. Thus injectable ^<15>O-O_2 with PET can be used to estimate oxygen metabolism reliably in stroke animal models, and may be useful for accelerating basic research on cerebral diseases.
- 2008-09-01
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関連論文
- Positron Emission Tomography (PET)イメージング法による小動物用インビボ局所脳酸素代謝率測定法の開発に関する研究
- マイクロメートルレベルでのインビボイメージングに挑戦する核医学装置(B・物理系薬学)