A Modified Coomassie Brilliant Blue G 250 Staining Method for the Detection of Chitinase Activity and Molecular Weight after Polyacrylamide Gel Electrophoresis(METHODS)

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概要

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• A modified Coomassie Brilliant Blue G 250 staining method for detecting chitinolytic enzymes in chitin-containing polyacrylamide gel electrophoresis (PAGE) is presented. The staining formed achromatic zones at the locations of the migrated enzyme. Using Streptomyces griseus chitinase, we have demonstrated that our method is more sensitive and less complicated than the conventional Calcofluor white M2R staining.

• 公益社団法人日本生物工学会の論文
• 2008-07-25

著者

• Liau Chun

  Institute Of Medicine Chung Shan Medical University:department Of Bioengineering Tatung University

• Lin Chung-Sheng

  Institute of Medicine, Chung Shan Medical University

• Lin Chung-sheng

  Institute Of Medicine Chung Shan Medical University

関連論文

• A Modified Coomassie Brilliant Blue G 250 Staining Method for the Detection of Chitinase Activity and Molecular Weight after Polyacrylamide Gel Electrophoresis(METHODS)

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