Genistein Protects Against Biomarkers of Delayed Lung Sequelae in Mice Surviving High-Dose Total Body Irradiation

スポンサーリンク

概要

• 論文の詳細を見る

• The effects of genistein on 30-day survival and delayed lung injury were examined in C57BL/6J female mice. A single subcutaneous injection of vehicle (PEG-400) or genistein (200 mg/kg) was administered 24 h before total body irradiation (7.75 Gy ^<60>Co, 0.6 Gy/min). Experimental groups were: No treatment + Sham (NC), Vehicle + Sham (VC), Genistein + Sham (GC), Radiation only (NR), Vehicle + Radiation (VR), Genistein + Radiation (GR). Thirty-day survivals after 7.75 Gy were: NR 23%, VR 53%, and GR 92%, indicating significant protection from acute radiation injury by genistein. Genistein also mitigated radiation-induced weight loss on days 13-28 postirradiation. First generation lung fibroblasts were analyzed for micronuclei 24 h postirradiation. Fibroblasts from the lungs of GR-treated mice had significantly reduced micronuclei compared with NR mice. Collagen deposition was examined by histochemical staining. At 90 days postirradiation one half of the untreated and vehicle irradiated mice had focal distributions of small collagen-rich plaques in the lungs, whereas all of the genistein-treated animals had morphologically normal lungs. Radiation reduced the expression of COX-2, transforming growth factor-β receptor (TGFβR) I and II at 90 days after irradiation. Genistein prevented the reduction in TGFβRI. However, by 180 days postirradiation, these proteins normalized in all groups. These results demonstrate that genistein protects against acute radiation-induced mortality in female mice and that GR-treated mice have reduced lung damage compared to NR or VR. These data suggest that genistein is protective against a range of radiation injuries.

• 日本放射線影響学会の論文
• 2008-07-16

著者

• LANDAUER MICHAEL

  Armed Forces Radiobiology Research Institute

• Davis Thomas

  Department Of Regenerative Medicine Naval Medical Research Center Silver Spring

• Davis Thomas

  Department Of Human Oncology And University Of Wisconsin Comprehensive Cancer Center

• Landauer Michael

  Armed Forces Radiobiology Research Institute Bethesda

• Barshishat-kupper Michal

  Department Of Pharmacology Uniformed Services University Of The Health Sciences Bethesda

• DAY Regina

  Department of Pharmacology Uniformed Services University of the Health Sciences Bethesda

• MOG Steven

  Armed Forces Radiobiology Research Institute Bethesda

• MCCART Elizabeth

  Armed Forces Radiobiology Research Institute Bethesda

• PRASANNA P.

  Armed Forces Radiobiology Research Institute Bethesda

関連論文

• RETINOBLASTOMA CONTROL PROTEINS(RCPs), Sp1, AND NFkB REGULATE X-RAY-INDUCIBLE TRANSCRIPTS IN ARRESTED HUMAN MELANOMA CELLS FOLLOWING IONIZING RADIATION
• Indomethacin Attenuation of Radiation-Induced Hyperthermia Does Not Modify Radiation-Induced Motor Hypoactivity
• Genistein Protects Against Biomarkers of Delayed Lung Sequelae in Mice Surviving High-Dose Total Body Irradiation
• Synthetic Trehalose Dicorynomycolate (S-TDCM) : Behavioral Effects and Radioprotection

スポンサーリンク