セレノシステイン生合成系とその種多様性
スポンサーリンク
概要
- 論文の詳細を見る
Selenium (Se) is an essential trace element. Se is found as selenocysteine (Sec) in Se-proteins. Sec is the 21st amino acid, because Sec has its tRNA, the codon UGA and those components in its translational machinery. Sec UGA codon shares with major stop codon UGA. We purified Sec synthesizing enzymes, such as seryl-tRNA synthetase (SerRS) , Sec synthetase (SecS) and selenophosphate synthetase (SePS). I described the procedures to prepare Sec tRNA, SerRS, SecS, SePS and [^<75>Se] H_2Se in detail. We clarified that SecS composed of two proteins, SecSα and SecSβ. Sec synthesizing and incorporating systems present in Monela, Animalia and Protoctista but not in Plantae and Fungi. We showed that protozoa had Sec tRNA on which Sec was synthesized from Ser-tRNA by bovine and protozoa SecS. Some worms, such as Caenorhabditis elegans and Fasiola gigantica, also had Sec tRNA on which Sec was synthesized by bovine liver SecS or C. elegans enzymes. We showed recognition sites of mammalian Sec tRNA by SecS. The identitiy units of Sec tRNA are 9 by aminoacyl- and 6 by D-stems. This recognition is not the base-specific manner but the length-specific manner. From comparison of the phylogeny trees of Sec synthesizing system and translation system, we concluded that the evolution of Sec synthesizing system is older than that of the translation system.
- 2008-07-01
著者
-
藤原 俊伸
神戸大院・工・応用化学
-
藤原 俊伸
神戸大学大学院工学研究科
-
藤原 俊伸
東大・医科研
-
大坂 享史
名古屋市立大学大学院薬学研究科医薬品代謝解析学分野
-
水谷 隆治
名古屋市立大学大学院薬学研究科医薬品代謝解析学分野
-
Shahidzzman M.
名古屋市立大学大学院薬学研究科医薬品代謝解析学分野
関連論文
- 2Gp13 メタボローム解析から捉えた線虫Caenorhabditis elegansのlife cycle(代謝工学,一般講演)
- RNA結合蛋白質が主役となる神経系での転写後遺伝子発現調節 (RNAと生命) -- (RNAと高次生命現象)
- 高度好熱菌Thermus thermophilus由来ribosome recycling factor遺伝子のクローニング
- セレノシステイン生合成系とその種多様性
- Sec UGA暗号と終止暗号の識別構造の新モデル
- 発生・分化と翻訳制御--RNA結合蛋白質がつかさどる高次生命現象 (第5土曜特集 RNA医学・医療--あらたな診断・治療を拓く) -- (RNA研究の新展開)
- 図表引用の許諾について
- タイトル無し