Pediocin PA-1 Production during Repeated-Cycle Batch Culture of Immobilized Pediococcus acidilactici UL5 Cells(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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Pediocin PA-1 production by Pediococcus addilactici UL5 cells immobilized in K-carrageenan/locust bean gum gel beads was studied during repeated-cycle batch(RGB) culture with pH control in Man Rogosa and Sharpe(MRS) broth supplemented with 1% glucose and whey permeate(SWP) medium. The pediocin PA-1 production by free P. addilactici cells pH-controlled batch culture has reached 2048 and 4096 AU ml^<-1> after 11 and 12 h of incubation, with volumetric productivities of 187 and 342 AU ml^<-1> h^<-1> in SWP and MRS media, respectively. In RGB culture, immobilized cells reached a maximum concentration of 7.3±0.2×10^<10> and 4.3±0.9×10^<10> cfu g^<-1> of beads in MRS and SWP media, respectively. The maximum pediocin PA-1 activity obtained during RGB fermentation was 4096 AU ml^<-1>; it was attained after only 0.75 and 2 h of incubation in MRS and SWP media, respectively. The corresponding volumetric productivities were 5461 and 2048 AU ml^<-1> h^<-1>. Pediocin PA-1 production in the RGB culture was highly stable over 12 fermentation cycles carried out over 3 d in SWP media.
公益社団法人日本生物工学会の論文
2008-05-25