P-54 ヒト複合型糖鎖をもつ小型糖タンパク質の化学合成(ポスター発表の部)

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Oligosaccharide chains on the protein play important roles for several biological events. However, the oligosaccharide exhibits heterogeneous structure, so called glycoform. Therefore synthesis of glycoprotein having intact and homogeneous oligosaccharide chains have been paid an attention in order to investigate what oligosaccharide structure on protein is essential for individual biological events. We have examined synthesis of glycoprotein having homogeneous human oligosaccharide and we presented the first chemical synthesis of glycoprotein, CC-Chemokine in the last of this meeting. Our synthetic strategy employed solid phase peptide synthesis and repetitive native chemical ligation method. We have examined synthesis of several small glycoproteins such as Ovomucoid and Crambin. In addition, we examined to evaluate an effect of oligosaccharide for protein folding process and protein conformation. We selected additional two targets, Ovomucoide and Crambin. Ovomucoid consists of 58 amino acids and it has three disulfide bonds. Crambin consists of 46 amino acids and it has also three disulfide bonds. We succeeded the synthesis of these glycosylated Ovomucoid and Crambin by use of our strategy. For glycosylated Ovomucoide, oligosaccharide was found to disturb protein folding process, but there was no interference during folding process in the case of glycosylated Crambin. We also succeeded measurement of the first TOCSY spectrum of glycoprotein synthesized. In this presentation, we would like to explain these interesting results in detail.
2007-08-24

P-54 ヒト複合型糖鎖をもつ小型糖タンパク質の化学合成(ポスター発表の部)

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