82(P73) 新規エンジイン抗生物質N1999A2の構造(ポスター発表の部)

概要

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In the course of our screening program for new antitumor antibiotics, the fermentation broth filtrate of Streptomyces sp. AJ9493 was found to produce active substance. The active compound, N1999A2, was purified by Diaion HP-20 column chromatography, ethyl acetate extraction, silica gel column chromatography, ODS column chromatography and preparative ODS HPLC. The methanolysis compound of N1999A2 was deduced to 1-naphthoate derivative by X ray crystal analysis and NMR assignments. The structure of N1999A2 was established by using variety of NMR technique, especially HMBC spectrum on several J values. N1999A2 was new enediyne antibiotic, similar to Neocarzinostatin chromophore (NCS-Chr). The major difference between N1999A2 and NCS-Chr was lack of the amino sugar in N1999A2, which was considered as the reason why N1999A2 was stable in spite of non-protein chromophore. N1999A2 was strongly inhibit the growth of tumor cell lines and bacteria. The IC_<50> of cytotoxicity to HCT-15 cell was 0.008nmol/ml.
天然有機化合物討論会の論文
1996-09-02