70 新規フラボノール配糖体 : タマネギ孔辺細胞の蛍光原因物質(ポスター発表の部)

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It has been well known that the guard cells of green leaves of Allium cepa (onion) showed an intrinsic green fluorescence under UV irradiation. However, the causal principal has not been characterized until now. Here, we report isolation and structure determination of the fluorescing compounds from the epidermal strips of green onion leaves. Two new flavonol glycosides were isolated from the aqueous methanol extract by using polyamide column, Sephadex LH-20 column and reverse phase HPLC as summarized in Scheme 1. These structures were determined to be kaempeferol -3-O-sophoroside-7-O-glucuronide 1 and quercetin-3-O-sophoroside-7-O-glucuronide 2 by ^1H and ^<13>C NMR (Table 1), UV (Figure 2), FAB Mass, and hydrolytic reactions (enzymic and acidic). From their fluorescence properties (Figure 3), 1 and 2 is seemed to be responsible for major fluorescing compounds in guard cells of Allium cepa. Particularly, the spectrum of 2 which is the most abundant flavonoid in the epidermis is almost identical with that of intact guard cell.
天然有機化合物討論会の論文
1991-09-07