46 微小管配向を制御する微生物代謝産物の化学的研究

概要

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In the continuing search for microbial cultures having cell proliferation inhibitors, it was found that a culture extract of Penicillium sp.A-5-11 showed pronounced activity against sea urchin embryo cells. The three active constituents, curvularin(1), dehydrocurvularin(2) and 8-methoxycurvularin(3) ,unknown, were so far isolated. These compounds showed very unique effects on spindle formation. The cells treated with 10^<-6>-10^<-5> M of the compounds gave barrel-like spindles and the first cleavage was completely blocked. Spindle poisons, colchicine and podophyllotoxin are known to disassemble microtuble of the developing embryos and prevent spindle formation. However, these three compounds possess different mode of operation. These chemicals disperse the microtuble organizing center and induce barrel-like spindles. The development of astral rays is blocked and chromosome movement is effectively prevented. Twenty compounds were obtained from partial modification of curvularin and dehydrocurvularin and the essential portion of curvularin molecule required for the induction of barrel-like spindle were studied. Dehydrocurvularin, 3'-methoxycurvularin and 10-deoxocurvularin were shown to be most active among the compounds tested.
天然有機化合物討論会の論文
1985-09-07