Oxygen Vectors Used for S-Adenosylmethionine Production in Recombinant Pichia pastoris with Sorbitol as Supplemental Carbon Source(BIOCHEMICAL ENGINEERING)

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概要

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• In order to increase the yield of S-adenosylmethionine(SAM) in recombinant Pichia pastoris, a strategy of adding oxygen vectors and supplemental carbon sources was described. Three organic solutions were used as oxygen vectors for SAM accumulation at different concentrations and addition times. Firstly, n-hexane(0.5%) or n-heptane(1.0%) was added after 72 h of cultivation to improve SAM production. Carbon metabolism was scarce during the induction phase because of low methanol concentration. Secondly, sorbitol(1.2%), selected from three candidates(glycerol, lactic acid, and sorbitol), was used as the supplemental carbon source. The yield of SAM was improved significantly(53.26%) at 1.0% w-heptane added at 72 h(48 h induction), 1.2% sorbitol added at 72, 96, and 120 h of cultivation and 1.0% methanol added every 24 h during cultivation.

• 社団法人日本生物工学会の論文
• 2008-04-25

著者

• Zhang Jian-guo

  State Key Laboratory Of Bioreactor Engineering College Of Biotechnology East China University Of Sci

• Wang Xue-dong

  State Key Laboratory Of Bioreactor Engineering College Of Biotechnology East China University Of Sci

• Zhang Ji-Ning

  State Key Laboratory of Bioreactor Engineering, College of Biotechnology, East China University of S

• Wei Dong-Zhi

  State Key Laboratory of Bioreactor Engineering, College of Biotechnology, East China University of S

• Wei Dong-zhi

  State Key Laboratory Of Bioreactor Engineering College Of Biotechnology East China University Of Sci

関連論文

• Oxygen Vectors Used for S-Adenosylmethionine Production in Recombinant Pichia pastoris with Sorbitol as Supplemental Carbon Source(BIOCHEMICAL ENGINEERING)

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