ヒト免疫不全ウイルス(HIV)感染・増殖に対するレチノイン酸の抑制効果
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概要
- 論文の詳細を見る
The inhibitory effect of all-trans-retinoic acid on human immunodeficiency virus (HIV) replication upon infection was studied quantitatively with the novel bioassay system using a HTLV-I-carrying human T-cell line, MT-4. The results were summarized as follow: 1) Appearance of HIV antigen was significantly reduced when the cells were treated with 10μg/ml of the compound after infection. When HIV specific plaque assay was performed to titrate the virus from the supernatant of culture treated with 10μg/ml of all-trans-retinoic acid no plaque was observed. 2) When all-trans-retinoic acid was used directly in the plaque assay, although control showed 102 plaque formation, significant decrease of the number of plaques was discerned showing 66, 47 and 16 at the concentration of 1, 5 and 10μg/ml of all-trans-retinoic acid respectively. 3) Cytopathic effect of MT-4 cells by HIV infection appeared in the all-trans-retinoic acid treated cultures later than in the untreated. 4) Concomitant treatment of the cells with 5μg/ml of all-trans-retinoic acid and various concentra tions of suramin resulted in the more effective inhibition of HIV replication. 5) all-trans-retinoic acid did not inhibit the reverse transcriptase activity of HIV directly. 6) DNA synthesis of MT-4 cells was inhibited when the cells were treated with all-trans-retinoic acid more than 0.1μg/ml with no significant effect on the cell viability. At the concentrations of 0.01μg/ml of all-trans-retinoic acid cellular DNA synthesis was rather enhanced. These data suggest that all-trans-retinoic acid inhibits HIV replication through its DNA inhibitory effect at the higher concentrations whereas it does by inducing antiviral state in the cells at the lower concentrations of all-trans-retinoic acid.
- 日本ビタミン学会の論文
- 1986-11-25