口腔白板症上皮細胞におけるマイコプラズマ感染 : 抗マイコプラズマ・ウサギポリクローナル抗体を用いた蛍光免疫組織化学による検討

概要

論文の詳細を見る
The etiology of oral leukoplakia has not been elucidated. A previous study using in situ staining with a DNA-binding fluorescent dye (Hoechst 33258) demonstrated numerous small granular structures in the cytoplasm of the epithelial cells in leukoplakia, suggesting that microorganisms were present. Furthermore, it was speculated that the microorganisms in the epithelial cells were oral mycoplasma. In this study, to examine intracellular infection by mycoplasma in epithelial cells with leukoplakia, fluorescent immunohistochemistry was performed using anti-mycoplasma rabbit polyclonal antibodies. Thirty-one formalin-fixed, paraffin-embedded specimens of leukoplakia without epithelial dysplasia and 19 epithelial dysplasia specimens were examined. Normal appearing mucosa specimens were used as controls. In all specimens of leukoplakia without epithelial dysplasia, numerous small granular fluorescing structures were observed within the cytoplasm of epithelial cells. In 10 of 19 (52.3%) specimens with epithelial dysplasia, fluorescing structures were observed, but were not observed in the remaining 9 specimens. In normal-appearing mucosa specimens, a few or no fluorescing structures were observed. These findings strongly suggest that mycoplasma infection is related to hyperkeratosis of oral mucosa. However, the relation between mycoplasma infection and epithelial dysplasia could not be clarified.
岩手医科大学歯学会の論文
2008-04-25